产青链霉菌重组菌株将青霉素G生物转化为去乙酰氧基头孢菌素G的性能。
Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G.
作者信息
Gao Q, Piret J M, Adrio J L, Demain A L
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
出版信息
J Ind Microbiol Biotechnol. 2003 Mar;30(3):190-4. doi: 10.1007/s10295-003-0034-4. Epub 2003 Mar 14.
We examined the performance of Streptomyces lividans strain W25 containing a hybrid expandase (deacetoxycephalosporin C synthase; DAOCS) gene, obtained by in vivo recombination between the expandase genes of S. clavuligerus and Nocardia lactamdurans for resting-cell bioconversion of penicillin G to deacetoxycephalosporin G. Strain W25 carried out a much more effective level of bioconversion than the previously used strain, S. clavuligerus NP1. The two strains also differed in the concentrations of FeSO(4) and alpha-ketoglutarate giving maximal activity. Whereas NP1 preferred 1.8 mM FeSO(4 )and 1.3 mM alpha-ketoglutarate, recombinant W25 performed best at 0.45 mM FeSO(4) and 1.9 mM alpha-ketoglutarate.
我们检测了含有杂合扩环酶（脱乙酰氧基头孢菌素C合酶；DAOCS）基因的淡紫链霉菌菌株W25的性能，该基因是通过棒状链霉菌和内酰胺诺卡氏菌的扩环酶基因之间的体内重组获得的，用于青霉素G的静息细胞生物转化为脱乙酰氧基头孢菌素G。菌株W25的生物转化水平比之前使用的菌株棒状链霉菌NP1要有效得多。这两种菌株在产生最大活性的硫酸亚铁和α-酮戊二酸浓度上也有所不同。NP1偏好1.8 mM硫酸亚铁和1.3 mMα-酮戊二酸，而重组菌株W25在0.45 mM硫酸亚铁和1.9 mMα-酮戊二酸时表现最佳。
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