聚（rA）和聚（rU）与变性DNA的结合。II. 天然DNA的研究。
The binding of poly (rA) and poly (rU) to denatured DNA. II. Studies with natural DNAs.
作者信息
Mol J N, Borst P
出版信息
Nucleic Acids Res. 1976 Apr;3(4):1029-51. doi: 10.1093/nar/3.4.1029.
Abstract
We have studied the interaction of poly(rA) and poly(rU) with natural DNAs containing (dA.dT)n sequences. The results indicate that hybridization of poly(rA) to denatured DNA can be used to estimate the size and frequency of large (dA.dT)n tracts, whereas hybridization with poly(rU) does not give reliable information on these points. In 6.6 M CsCl, poly(rU) can form stable complexes with denatured DNA containing short (dA)n tracts (n less than or equal to 6), whereas binding of poly(rA) to denatured DNA under these conditions requires much larger (dT)n tracts (estimated n greater than 13). Moreover, binding of poly(rA) requires pre-hybridization in low salt, because free poly(rA) precipitates in 6.6 M CsCl.
摘要
我们研究了聚（rA）和聚（rU）与含有（dA.dT）n序列的天然DNA之间的相互作用。结果表明，聚（rA）与变性DNA的杂交可用于估计大的（dA.dT）n片段的大小和频率，而与聚（rU）杂交在这些方面并不能提供可靠信息。在6.6M CsCl中，聚（rU）可与含有短（dA）n片段（n小于或等于6）的变性DNA形成稳定复合物，而在这些条件下聚（rA）与变性DNA的结合则需要大得多的（dT）n片段（估计n大于13）。此外，聚（rA）的结合需要在低盐中进行预杂交，因为游离的聚（rA）会在6.6M CsCl中沉淀。
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