Determination of the antigen binding capacity of antisera to collagen by gel filtration.
作者信息
Menzel J
出版信息
Z Immunitatsforsch Exp Klin Immunol. 1975 May;149(1):9-19.
PMID:127460
Abstract
Significant differences in the antigen binding capacity of different antisera to collagen of identical haemagglutination titres were demonstrated by using gel filtration for the isolation of collagen-anticollagen immune-complexes. A partially degraded collagen was used as the antigen. The influence of antigen concentration on antigen binding capacity was investigated with 2 antisera of different avidity. The distribution of antibody-bound antigen among IgM- and IgG- peaks indicates the participation of both immunoglobulin classes in the formation of collagen-anticollagen immune-complexes.
摘要
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