用于检测犬细小病毒和犬瘟热病毒免疫球蛋白M抗体的斑点酶联免疫吸附测定试剂盒的评估
Evaluation of a dot ELISA kit for measuring immunoglobulin M antibodies to canine parvovirus and distemper virus.
作者信息
Waner T, Mazar S, Nachmias E, Keren-Kornblatt E, Harrus S
机构信息
Israel Institute for Biological Research, PO Box 19, Ness Ziona, 70400, Israel.
出版信息
Vet Rec. 2003 May 10;152(19):588-91. doi: 10.1136/vr.152.19.588.
A dot ELISA for the detection of immunoglobulin M (IgM) antibodies to canine distemper virus (CDC) and canine parvovirus (CPV) was assessed. The titres of IgM antibodies to CDV and CPV in 100 dogs were measured by the Immunocomb ELISA kit and compared with the results derived from the immunofluorescence assay (IFA). There was a strong correlation between the results of the dot ELISA technique and the IFA (P < 0.001). The dot ELISA kit was also used to assess the changes in the levels of immunoglobulin G (IgG) and IgM antibodies to CPV and CDV in 10 puppies vaccinated with a polyvalent vaccine. High levels of IgM antibodies to CPV were first detected seven days after they were vaccinated, and after nine days all the pups had high titres of IgG antibodies to CPV. High levels of IgM antibodies to CDV were detected after nine days and the highest average titres were recorded after 12 days. IgG antibodies to CDV were present from nine days after vaccination.
对一种用于检测犬瘟热病毒（CDC）和犬细小病毒（CPV）免疫球蛋白M（IgM）抗体的斑点酶联免疫吸附测定（dot ELISA）进行了评估。采用免疫组合酶联免疫吸附测定试剂盒测定了100只犬中针对CDV和CPV的IgM抗体效价，并与免疫荧光测定（IFA）结果进行比较。斑点酶联免疫吸附测定技术结果与IFA结果之间存在强相关性（P < 0.001）。斑点酶联免疫吸附测定试剂盒还用于评估10只接种多价疫苗幼犬中针对CPV和CDV的免疫球蛋白G（IgG）和IgM抗体水平的变化。接种疫苗7天后首次检测到针对CPV的高水平IgM抗体，9天后所有幼犬针对CPV均具有高效价IgG抗体。接种疫苗9天后检测到针对CDV的高水平IgM抗体，12天后记录到最高平均效价。接种疫苗9天后出现针对CDV的IgG抗体。
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