Inoguchi Masahiko, Ogawa Satoshi, Furukawa Sanae, Kondo Hirokiyo
Department of Biochemistry, Okayama University of Science, 1-1 Ridai-cho, Okayama 700-0005, Japan.
Biosci Biotechnol Biochem. 2003 Apr;67(4):863-8. doi: 10.1271/bbb.67.863.
Hairy roots of goldenrod (Solidago altissima L.) were induced by infecting axenic plants with Agrobacterium rhizogenes strain A4. Growth and allelopathic polyacetylene (cis-dehydromatricaria ester, cis-DME) production of two independent hairy root clones were examined in several culture media and light regimes. cis-DME contents in hairy roots were at the same level as those in normal roots. cis-DME production in root cultures was several-fold lower than that of native plants and greatly repressed by light.
通过用发根农杆菌菌株A4感染无菌植物来诱导一枝黄花(Solidago altissima L.)的毛状根。在几种培养基和光照条件下检测了两个独立毛状根克隆的生长和化感聚乙炔(顺式脱氢母菊酯,cis-DME)的产生。毛状根中顺式-DME的含量与正常根中的含量处于同一水平。根培养物中顺式-DME的产量比原生植物低几倍,并且受到光照的极大抑制。