Isolation of human platelets (thrombocytes).
作者信息
Graham John M
机构信息
School of Biomolecular Sciences, Liverpool John Moores University.
出版信息
ScientificWorldJournal. 2002 Jun 12;2:1607-9. doi: 10.1100/tsw.2002.820.
Abstract
Platelets from human blood can be isolated in high yield by centrifugation of whole blood over an iodixanol density barrier of 1.063 g/ml. The separation from all of the blood cells (which form a pellet) is based on the slower sedimentation velocity of the smaller platelets.
摘要
相似文献
1
Isolation of human platelets (thrombocytes).
ScientificWorldJournal. 2002 Jun 12;2:1607-9. doi: 10.1100/tsw.2002.820.
2
Isolation of peripheral blood mononuclear cells from macaques on a density barrier.
ScientificWorldJournal. 2002 Jun 15;2:1654-6. doi: 10.1100/tsw.2002.845.
3
Flow cytometric analysis of CD41-labeled platelets isolated by the rapid, one-step OptiPrep method from human blood.
Cytometry A. 2005 May;65(1):84-7. doi: 10.1002/cyto.a.20133.
4
Isolation of human polymorphonuclear leukocytes (granulocytes) from a leukocyte-rich fraction.
ScientificWorldJournal. 2002 May 21;2:1393-6. doi: 10.1100/tsw.2002.831.
5
Isolation of rat and human hippocampal neuron fractions in a discontinuous density gradient.
ScientificWorldJournal. 2002 Jun 14;2:1634-7. doi: 10.1100/tsw.2002.850.
6
A new, rapid, one-step method for the isolation of platelets from human blood.
Clin Chim Acta. 1990 Nov 30;192(2):115-9. doi: 10.1016/0009-8981(90)90075-4.
7
Purification of gastric mucosal ECL cells from a crude elutriation fraction.
ScientificWorldJournal. 2002 Jun 14;2:1643-5. doi: 10.1100/tsw.2002.852.
8
Density separation of quiescent yeast using iodixanol.
Biotechniques. 2017 Oct 1;63(4):169-173. doi: 10.2144/000114596.
9
An efficient method for isolation of representative and contamination-free population of blood platelets for proteomic studies.
Platelets. 2017 Jan;28(1):43-53. doi: 10.1080/09537104.2016.1209478. Epub 2016 Sep 2.
引用本文的文献
1
Analysis of Cellular Stress Assay Parameters and Intracellular ATP in Platelets: Comparison of Platelet Preparation Methods.
Int J Mol Sci. 2024 Apr 30;25(9):4885. doi: 10.3390/ijms25094885.
2
Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes.
Nat Microbiol. 2016 Jun 20;1(9):16087. doi: 10.1038/nmicrobiol.2016.87.
3
Ultra-pure platelet isolation from canine whole blood.
BMC Vet Res. 2013 Jul 17;9:144. doi: 10.1186/1746-6148-9-144.
Zotero 插件