Alghanim H J, Almirall J R
Department of Chemistry, International Research Forensic Institute, Florida International University, University Park, Miami, FL 33314, USA.
Anal Bioanal Chem. 2003 Aug;376(8):1225-33. doi: 10.1007/s00216-003-1984-0. Epub 2003 Jun 13.
Microsatellite markers were developed for Cannabis sativa L. (marijuana) to be used for DNA typing (genotype identification) and to measure the genetic relationships between the different plants. Twelve different oligonucleotide probes were used to screen an enriched microsatellite library of Cannabis sativa in which 49% of the clones contained microsatellite sequences. Characterization of microsatellite loci in Cannabis revealed that GA/CT was the most abundant class of the isolated microsatellites representing 50% overall followed by GTT/CAA, AAG/TTC, and GAT/CTA representing 16%, 15%, and 10%, respectively. Eleven polymorphic STR markers were developed, three derived from dinucleotide motifs and eight from trinucleotide motifs. A total of 52 alleles were detected averaging 4.7 alleles/locus. The expected heterozygosity of the eleven loci ranged between 0.368 and 0.710 and the common probability of identical genotypes was 1.8 x 10(-7). The loci identified 27 unique profiles of the 41 Cannabis samples. The 11 microsatellite markers developed in this study were found to be useful for DNA typing and for assessing genetic relatedness in Cannabis.
开发了用于大麻（Cannabis sativa L.）的微卫星标记，用于DNA分型（基因型鉴定）并测量不同植株之间的遗传关系。使用12种不同的寡核苷酸探针筛选了大麻的富集微卫星文库，其中49%的克隆含有微卫星序列。对大麻微卫星位点的表征显示，GA/CT是分离出的微卫星中最丰富的类别，占总数的50%，其次是GTT/CAA、AAG/TTC和GAT/CTA，分别占16%、15%和10%。开发了11个多态性STR标记，其中3个来自二核苷酸基序，8个来自三核苷酸基序。总共检测到52个等位基因，平均每个位点4.7个等位基因。11个位点的预期杂合度在0.368至0.710之间，相同基因型的共同概率为1.8×10⁻⁷。这些位点在41个大麻样本中鉴定出27种独特的图谱。本研究中开发的11个微卫星标记被发现可用于大麻的DNA分型和评估遗传相关性。
