与固氮菌属FA8中假定的聚羟基丁酸酯调控基因相关的类插入序列元件。
Insertion sequence-like elements associated with putative polyhydroxybutyrate regulatory genes in Azotobacter sp. FA8.
作者信息
Pettinari Julia M, Chaneton Luciano, Vazquez Gustavo, Steinbüchel Alexander, Méndez Beatriz S
机构信息
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina.
出版信息
Plasmid. 2003 Jul;50(1):36-44. doi: 10.1016/s0147-619x(03)00009-x.
The genes phaR, phaP, and phaF, encoding putative regulatory proteins, were found in the poly (3-hydroxybutyrate) (PHB) gene cluster of the free nitrogen-fixing bacteria Azotobacter sp. FA8. These genes were flanked by the insertion sequence ISAzsp1, belonging to the IS3 family, and a region highly homologous to insertion sequences of the IS630 family. These are the first site-specific recombination elements to be described in association with genes involved in the metabolism of polyhydroxyalkanoates (PHAs). A possible role for ISs in the assembly of pha genes is presented.
在自生固氮菌固氮菌属FA8的聚（3-羟基丁酸酯）（PHB）基因簇中发现了编码假定调控蛋白的phaR、phaP和phaF基因。这些基因两侧是属于IS3家族的插入序列ISAzsp1，以及与IS630家族插入序列高度同源的区域。这些是首次报道的与聚羟基脂肪酸酯（PHA）代谢相关基因有关的位点特异性重组元件。文中提出了插入序列在pha基因组装中的可能作用。
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