丙型肝炎病毒抗体检测：c100-3酶免疫测定法与第二代酶免疫测定法的比较
Detection of antibodies to HCV: comparison of a c100-3 EIA and the 2nd generation EIA.
作者信息
Polywka S, Kaars-Wiele P, Schroeter E, Mandler J, Laufs R
机构信息
Institute of Medical Microbiology and Immunology, University Hospital Eppendorf, Hamburg, FRG.
出版信息
Beitr Infusionsther. 1992;30:42-5.
The reactivity of 558 serum specimens in Abbott 1st and 2nd generation EIAs was compared. A significant increase in HCV seropositivity was especially found in high risk groups such as hemophiliacs (57.7 vs. 73.1%), IVDA (54.5 vs. 61.8%), HIV positives (21.8 vs. 25.5%) or patients on dialysis (15.7 vs. 16.9%). Reactivity of all sera which only reacted in the 2nd generation EIA was 'confirmed' by using supplemental research assays. In contrast, sera which were highly reactive in the c100-3 assay but could not be confirmed by Neutralization Assay were weakly positive or negative in the 2nd generation test.
比较了558份血清标本在雅培第一代和第二代酶免疫测定（EIA）中的反应性。在血友病患者（57.7%对73.1%）、静脉注射毒品者（IVDA，54.5%对61.8%）、HIV阳性者（21.8%对25.5%）或透析患者（15.7%对16.9%）等高风险组中，尤其发现丙型肝炎病毒（HCV）血清阳性率显著增加。通过使用补充研究检测方法，“确认”了所有仅在第二代EIA中发生反应的血清的反应性。相比之下，在c100 - 3检测中反应性高但无法通过中和试验确认的血清，在第二代检测中呈弱阳性或阴性。
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