Büch U, Isenberg J C, Baldauf J, Altmayer P, Büch H P
Institut für Pharmakologie und Toxikologie, Klinik für Anästhesie und Intensivmedizin, Universität des Saarlandes, Homburg/Saar, Germany.
Methods Find Exp Clin Pharmacol. 1992 Sep;14(7):499-505.
Thiopental uptake into heart muscle tissue was studied in spontaneously beating rat hearts (Langendorff preparation, 0.13-0.27 mmol/l thiopental in the perfusion fluid). Up to 0.19 mmol/l the concentration of thiopental in heart muscle tissue was increased vs. control when halothane (0.8 vol%) was present. Using a constant thiopental concentration (0.13 mmol/l) and 0.8, 1.5 or 2.0 vol% halothane +12%, +29% or +43% more thiopental was taken up into heart muscle tissue compared to the control. This increased uptake was not seen in the presence of 1.2 and 2.0 vol% isoflurane. Frequency of right rat atria was decreased by increasing thiopental concentrations (0.02-0.23 mmol/l in the incubation medium). Halothane (0.8 and 1.5 vol%) and isoflurane (1.0 and 2.0 vol%) alone had no influence on frequency of right atria. Both volatile anesthetics additionally increased the negative chronotropic action of thiopental when the corresponding higher concentration was applied. Contractile force of left rat atria was decreased concentration-dependently by thiopental (0.02-0.23 mmol/l). Halothane and isoflurane alone decreased contractility. Dependent on the concentration used, both volatile anesthetics further increased the negative inotropic action of thiopental, yet preferentially at higher barbiturate concentrations.
在自发搏动的大鼠心脏（Langendorff 制备法，灌注液中硫喷妥钠浓度为 0.13 - 0.27 mmol/l）中研究了硫喷妥钠在心肌组织中的摄取情况。当存在氟烷（0.8 体积%）时，心肌组织中硫喷妥钠浓度在达到 0.19 mmol/l 之前相对于对照组有所增加。使用恒定的硫喷妥钠浓度（0.13 mmol/l），与对照组相比，0.8、1.5 或 2.0 体积%的氟烷会使心肌组织摄取的硫喷妥钠增加 12%、29%或 43%。在 1.2 和 2.0 体积%异氟烷存在的情况下未观察到这种摄取增加的情况。增加孵育培养基中硫喷妥钠的浓度（0.02 - 0.23 mmol/l）会降低右心房大鼠的心率。单独使用氟烷（0.8 和 1.5 体积%）和异氟烷（1.0 和 2.0 体积%）对右心房心率没有影响。当使用相应较高浓度时，两种挥发性麻醉剂都会进一步增强硫喷妥钠的负性变时作用。硫喷妥钠（0.02 - 0.23 mmol/l）浓度依赖性地降低左心房大鼠的收缩力。单独使用氟烷和异氟烷会降低收缩性。根据所使用的浓度，两种挥发性麻醉剂都会进一步增强硫喷妥钠的负性变力作用，但在较高的巴比妥酸盐浓度下更为明显。
