Determination of bupivacaine and metabolites in rat urine using capillary electrophoresis with mass spectrometric detection.
作者信息
Krisko Ryan M, Schieferecke Mark A, Williams Todd D, Lunte Craig E
机构信息
Department of Chemistry, University of Kansas, Lawrence, KS, USA.
出版信息
Electrophoresis. 2003 Jul;24(14):2340-7. doi: 10.1002/elps.200305469.
Abstract
A method using capillary electrophoresis-mass spectrometry (CE-MS) was developed for the structural elucidation of bupivacaine and metabolites in rat urine. Prior to CE-MS analysis, solid-phase extraction (SPE) was used for sample cleanup and preconcentration purposes. Exact mass and tandem mass spectrometric (MS/MS) experiments were performed to obtain structural information about the unknown metabolites. Two instruments with different mass analyzers were used for mass spectrometric detection. A quadrupole time-of-flight (Q-TOF) and a magnetic sector hybrid instrument were coupled to CE and used for the analysis of urine extracts. Hydroxybupivacaine as well as five other isomerically different metabolites were detected including methoxylated bupivacaine.
摘要
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