一种基于铂电镀碳电极的生物相容性针型葡萄糖传感器。

A biocompatible needle-type glucose sensor based on platinum-electroplated carbon electrode.

作者信息

Chen C Y, Tamiya E, Ishihara K, Kosugi Y, Su Y C, Nakabayashi N, Karube I

机构信息

Research Center for Advanced Science and Technology, University of Tokyo, Japan.

出版信息

Appl Biochem Biotechnol. 1992 Sep;36(3):211-26. doi: 10.1007/BF02921780.

DOI:10.1007/BF02921780

PMID:1288410

Abstract

A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a disposable hypodermic needle made of stainless steel as the counter electrode. A Nafion membrane, an immobilized glucose oxidase (GOD) membrane, and a biocompatible membrane with diffusion-limiting effect were coated successively onto the working electrode. The sensor showed a rapid response (< 120 s in batch operation), good reproducibility (RE < 3%), good stability (over 36 h in control serum), a wide dynamic range (5-600 mg/dL glucose), and superior biocompatibility. It was used to determine glucose in serum. The data obtained from the sensor showed good agreement with that from a clinical autoanalyzer (R > 0.95).

摘要

构建了一种具有三电极配置的生物相容性针型葡萄糖传感器。镀铂碳棒用作工作电极，Ag/AgCl用作参比电极，不锈钢制成的一次性皮下注射针用作对电极。将Nafion膜、固定化葡萄糖氧化酶（GOD）膜和具有扩散限制作用的生物相容性膜依次涂覆在工作电极上。该传感器显示出快速响应（批量操作中<120秒）、良好的重现性（相对误差<3%）、良好的稳定性（在对照血清中超过36小时）、宽动态范围（5 - 600mg/dL葡萄糖）以及优异的生物相容性。它用于测定血清中的葡萄糖。从该传感器获得的数据与临床自动分析仪的数据显示出良好的一致性（R>0.95）。