Mase Masaji, Yamaguchi Shigeo, Tsukamoto Kenji, Imada Tadao, Imai Kunitoshi, Nakamura Kikuyasu
Department of Infectious Diseases, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Avian Dis. 2003 Apr-Jun;47(2):481-4. doi: 10.1637/0005-2086(2003)047[0481:POAPSA]2.0.CO;2.
Four avian pneumovirus (APV) isolates from chickens clinically diagnosed with swollen head syndrome were genetically characterized as to the subtypes of the virus in Japan. The results of reverse transcriptase-polymerase chain reactions based on subtype-specific primers and direct sequence analysis of G genes indicated subtypes A and B but not C or D of APV were present in Japan. Several routes or sources are conceivable for APV to invade into Japan.
对从临床上诊断为肿头综合征的鸡中分离出的4株禽肺病毒(APV)进行基因特征分析,以确定其在日本的病毒亚型。基于亚型特异性引物的逆转录聚合酶链反应结果以及G基因的直接序列分析表明,日本存在APV的A和B亚型,但不存在C或D亚型。APV侵入日本可能有几种途径或来源。