[Cisplatin-induced apoptotic cell death in spiral ganglion and organ of Corti of mongolian gerbil cochlear].

OBJECTIVE

To investigate whether apoptosis is one of the mechanism for cell death in spiral ganglion(SG) and organ of Corti of Mongolian gerbils cochlea induced by cisplatin.

METHODS

Mongolian gerbils were administered 4 mg.kg-1.d-1 cisplatin consecutively for 4 to 7 days. The apoptotic cell death in spiral ganglion and organ of Corti of cochlea basal turn was detected via transmission electron microscopy and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method.

RESULTS

The observation of electron microscopy shown special morphological change consistent with the feature of cell apoptosis in some spiral ganglion cells and outer hair cells after 5-7 days' cisplatin treatment. The apoptotic labeling by TUNEL in spiral ganglion and organ of Corti was detected after 5 days' treatment and increased after 6 and 7 days' treatment whereas almost negative result were obtained in the normal control animals.

CONCLUSION

Apoptosis is one of the important mechanisms for cell injury of spiral ganglion and organ of Corti induced by cisplatin.