使用与聚阳离子梳型共聚物结合的寡核苷酸进行三链体形成。

Triplex formation using ODN conjugates with polycation comb-type copolymer.

作者信息

Ueda M, Saito M, Ishihara T, Akaike T, Maruyama A

机构信息

Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa 226-8501, Japan.

出版信息

Nucleic Acids Symp Ser. 2000(44):209-10. doi: 10.1093/nass/44.1.209.

DOI:10.1093/nass/44.1.209

PMID:12903342

Abstract

Polycation comb-type copolymer that is composed of polylysine backbone and dextran side chains (PLL-g-Dex) has previously been shown to stabilize duplex and triplex DNAs quite effectively. In this study, we have conjugated PLL-g-Dex with oligonucleotides (ODN) aiming to increase the triplex stabilizing efficiency of the copolymer. Here we have demonstrated that the copolymer-TFO conjugates selectively stabilize triplex DNA. Also its potential to form triplex DNA was found to be greater than PLL-g-Dex/ODN mixture.

摘要

由聚赖氨酸主链和葡聚糖侧链组成的聚阳离子梳型共聚物（PLL-g-Dex）此前已被证明能非常有效地稳定双链和三链DNA。在本研究中，我们将PLL-g-Dex与寡核苷酸（ODN）偶联，旨在提高该共聚物的三链稳定效率。在此我们证明了共聚物-TFO偶联物能选择性地稳定三链DNA。此外，还发现其形成三链DNA的潜力大于PLL-g-Dex/ODN混合物。