候选人类乳头瘤病毒92型的核苷酸序列及系统发育分类

Nucleotide sequence and phylogenetic classification of candidate human papilloma virus type 92.

作者信息

Forslund Ola, Antonsson Annika, Higgins Geoff, Ly Hoang, Delius Hajo, Hunziker Andreas, de Villiers Ethel Michele

机构信息

Department of Medical Microbiology, Malmö University Hospital, Lund University, Malmö, Sweden.

出版信息

Virology. 2003 Aug 1;312(2):255-60. doi: 10.1016/s0042-6822(03)00391-x.

DOI:10.1016/s0042-6822(03)00391-x

PMID:12919731

Abstract

From a basal cell carcinoma (BCC) the complete genome of candidate human papillomavirus (HPV) type 92 was characterized. Phylogenetically, the candidate HPV 92 was relatively distantly related to other cutaneous HPV types within the B1 group. By quantitative real time PCR, 94 viral copies were present per cell in the BCC and another BCC contained 1 viral copy per cell. Lower copy numbers were found in two solar keratoses (1 copy per 33 cells and 1 copy per 60 cells) and two squamous cell carcinomas (1 copy per 436 cells and 1 copy per 1143 cells). The high viral load of HPV 92 in two BCCs differs from the low amount of HPV DNA reported from nonmelanoma skin cancers.

摘要

对来自基底细胞癌（BCC）的候选人类乳头瘤病毒（HPV）92型的全基因组进行了特征分析。从系统发育角度看，候选HPV 92与B1组内的其他皮肤HPV类型亲缘关系相对较远。通过定量实时PCR检测，在基底细胞癌中每个细胞存在94个病毒拷贝，另一个基底细胞癌中每个细胞含有1个病毒拷贝。在两个日光性角化病（每33个细胞1个拷贝和每60个细胞1个拷贝）和两个鳞状细胞癌（每436个细胞1个拷贝和每1143个细胞1个拷贝）中发现了较低的拷贝数。两个基底细胞癌中HPV 92的高病毒载量与非黑素瘤皮肤癌中报道的低HPV DNA量不同。