雌雄异株的欧洲树木枸骨叶冬青（英国冬青）的叶绿体DNA多样性。

Chloroplast DNA diversity of the dioecious European tree Ilex aquifolium L. (English holly).

作者信息

Rendell S, Ennos R A

机构信息

Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.

出版信息

Mol Ecol. 2003 Oct;12(10):2681-8. doi: 10.1046/j.1365-294x.2003.01934.x.

DOI:10.1046/j.1365-294x.2003.01934.x

PMID:12969471

Abstract

Variation in the chloroplast genome of Ilex aquifolium (English holly), a dioecious evergreen tree native to south, west and central Europe, was analysed using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) and microsatellites. Differentiation between populations was high (GST = 0.595) and evidence for phylogeographical structure was detected (NST = 0.697, significantly higher than GST). Two chloroplast lineages were inferred originating from putative glacial refugia in southern Europe (Iberia, Italy and possibly the Balkans). The GST value was higher than reported for endozoochorous hermaphrodite species and for anemochorous dioecious species investigated over a similar geographical scale. It appears that dioecy has contributed to strong differentiation between refugia and that this has been maintained following postglacial recolonization as a result of limited seed flow. Palynological records for I. aquifolium are poor, thus these results give an important insight into patterns of glacial isolation and postglacial recolonization of this species.

摘要

利用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和微卫星分析了欧洲冬青（Ilex aquifolium）叶绿体基因组的变异情况。欧洲冬青是一种雌雄异株的常绿乔木，原产于欧洲南部、西部和中部。种群间的分化程度很高（GST = 0.595），并且检测到了系统地理学结构的证据（NST = 0.697，显著高于GST）。推断出两个叶绿体谱系起源于欧洲南部的假定冰川避难所（伊比利亚半岛、意大利以及可能的巴尔干半岛）。GST值高于在类似地理尺度上研究的内寄生雌雄同体物种和风媒雌雄异株物种的报道值。看来雌雄异株导致了避难所之间的强烈分化，并且由于种子传播有限，这种分化在冰期后重新定殖后得以维持。欧洲冬青的孢粉学记录很少，因此这些结果为该物种的冰期隔离模式和冰期后重新定殖提供了重要的见解。