UNGAR G, DAMGAARD E, HUMMEL F P
J Exp Med. 1953 Oct;98(4):291-303. doi: 10.1084/jem.98.4.291.
The activation of profibrinolysin in sensitized guinea pig serum when mixed in vitro with the homologous antigen was confirmed with a more accurate and more reliable method than the one previously used. A study was made of some of the conditions required for obtaining maximum activation. Profibrinolysin activation was also induced in normal guinea pig serum by addition of certain "anaphylactoid" agents such as peptone, tween 20, morphine, octylamine, octadecylamine, and 48/80. The specific antigen and the anaphylactoid agents produce activation only when added to whole, fresh, unheated serum. Profibrinolysin activation by these agents, as opposed to activation by streptokinase, seems to require the intervention of a kinase system (serofibrinokinase) inactivated by fractionation of serum and by heating to 56 degrees C. Whenever serum was submitted to treatments which caused fractionation, fixation or inhibition of complement, serofibrinokinase was also inactivated. Under the conditions investigated the behavior of this kinase was indistinguishable from that of complement.
与先前使用的方法相比,采用一种更精确、更可靠的方法证实了在体外将致敏豚鼠血清与同源抗原混合时,纤溶酶原激活物的激活情况。对获得最大激活所需的一些条件进行了研究。通过添加某些“类过敏反应”剂,如蛋白胨、吐温20、吗啡、辛胺、十八胺和48/80,也可在正常豚鼠血清中诱导纤溶酶原激活。特异性抗原和类过敏反应剂只有在添加到完整、新鲜、未加热的血清中时才会产生激活作用。与链激酶激活不同,这些试剂激活纤溶酶原似乎需要一种激酶系统(血清纤维蛋白激酶)的参与,该系统会因血清分级分离和加热至56℃而失活。每当血清接受导致分级分离、固定或抑制补体的处理时,血清纤维蛋白激酶也会失活。在所研究的条件下,这种激酶的行为与补体的行为无法区分。