组织培养研究；鸡胚提取物可超滤部分中活性成分的分离。

Tissue culture studies; isolation of the active components in the ultrafilterable portion of the chick embryo extract.

作者信息

ROSENBERG S, ZITCER E, KIRK P L

出版信息

J Gen Physiol. 1953 Nov 20;37(2):231-8. doi: 10.1085/jgp.37.2.231.

DOI:10.1085/jgp.37.2.231

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2147430/

Abstract

A fraction of the ultrafilterable portion of chick embryo extract was isolated by alcohol extraction of a lyophilized powder of the ultrafiltrate followed by ion exchange removal of many of the inert components of the alcohol extract. This fraction contained 3 per cent of the ultrafilterable nitrogen but was capable of completely restoring the growth-promoting activity of dialyzed embryo extract, when tested with chick heart fibroblasts in roller tube cultures. The low nitrogen content, shape of the ultraviolet absorption spectrum, and presence of few free amino acids, suggest that non-dialyzable compounds serve as the chief source of nutrition for this system.

摘要

通过对超滤物冻干粉末进行乙醇提取，随后用离子交换法去除乙醇提取物中的许多惰性成分，从而分离出鸡胚提取物超滤部分的一小部分。当在滚瓶培养中用鸡心脏成纤维细胞进行测试时，该部分含有3%的可超滤氮，但能够完全恢复透析胚胎提取物的促生长活性。低氮含量、紫外吸收光谱的形状以及游离氨基酸含量较少，表明不可透析的化合物是该系统的主要营养来源。

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引用本文的文献

1

Tissue culture studies; identification of components and synthetic replacements for the active fraction of chick embryo extract ultrafiltrate.

J Gen Physiol. 1953 Nov 20;37(2):239-48. doi: 10.1085/jgp.37.2.239.

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