Liebler E M, Klüver S, Pohlenz J, Koopmans M
Institut für Pathologie, Tierärztlichen Hochschule Hannover.
Dtsch Tierarztl Wochenschr. 1992 May;99(5):195-200.
The objective of this investigation was to determine the distribution of Bredavirus in cattle herds in Lower Saxony and to evaluate its significance as potential cause of diarrhea in calves. Fecal samples and paired blood samples of 119 diarrheic and 46 healthy calves up to two months of age were collected from herds where diarrhea of calves was a problem. Fecal samples were examined for Breda-, rota- and coronavirus by solid phase immune electron microscopy and by ELISA, for K99-positive E. coli and salmonella by microbiological methods, and for cryptosporidia in smears. Antibody titers against Bredavirus, total serum protein and serum gamma globulin content were evaluated in the blood samples. Bredavirus was found in fecal samples from 5% (n = 6) of diarrheic calves which came from four different herds, but not in healthy calves. Rotavirus (31.9%), coronavirus (18.5%) and cryptosporidia (29.9%) were detected more frequently in fecal samples than Bredavirus. In this investigation rotavirus, coronavirus and cryptosporidia were present in addition in all herds where Bredavirus was found. In contrast to the low percentage of fecal samples containing Bredavirus, antibody titers in 75% of calves confirmed the high prevalence of Bredavirus infection in the cattle population of Lower Saxony.
本调查的目的是确定下萨克森州牛群中布雷达病毒的分布情况,并评估其作为犊牛腹泻潜在病因的重要性。从犊牛腹泻成问题的牛群中收集了119头腹泻犊牛和46头2月龄以下健康犊牛的粪便样本及配对血液样本。粪便样本通过固相免疫电子显微镜和酶联免疫吸附测定法检测布雷达病毒、轮状病毒和冠状病毒,通过微生物学方法检测K99阳性大肠杆菌和沙门氏菌,并通过涂片检测隐孢子虫。在血液样本中评估针对布雷达病毒的抗体滴度、总血清蛋白和血清γ球蛋白含量。在来自四个不同牛群的5%(n = 6)腹泻犊牛的粪便样本中发现了布雷达病毒,但在健康犊牛中未发现。粪便样本中轮状病毒(31.9%)、冠状病毒(18.5%)和隐孢子虫(29.9%)的检出频率高于布雷达病毒。在本调查中,在发现布雷达病毒的所有牛群中还存在轮状病毒、冠状病毒和隐孢子虫。与含有布雷达病毒的粪便样本比例较低形成对比的是,75%犊牛的抗体滴度证实下萨克森州牛群中布雷达病毒感染的高流行率。