Baldacci P A, Richoux V, Renard J P, Guénet J L, Babinet C
Unité de Génétique des Mammifères, Institut Pasteur, Paris, France.
Mamm Genome. 1992;2(2):100-5. doi: 10.1007/BF00353857.
The DDK inbred strain of mouse has a striking particularity: when DDK females are crossed to males of other strains they exhibit a reduced fertility, whereas the reciprocal crosses (non-DDK females x DDK males) are fertile (Wakasugi et al. 1967; Wakasugi 1973). The low fertility results from an early embryonic lethality, the F1 embryos dying near the late morula-early blastocyst stage. Genetic analyses (Wakasugi 1974) and nuclear and cytoplasmic transfers (Renard and Babinet 1986; Babinet et al. 1990; Mann 1986), have shown that the failure of the embroys to develop is due to an incompatibility between a DDK maternally encoded cytoplasmic product and the non-DDK paternal genome. In order to elucidate the genetic determinism of this embryonic lethality, we have analyzed the fertility of male progeny from a backcross BALB/c females x (BALB/c x DDK)F1 males and that of males from a set of recombinant inbred (RI) strains, established from DDK and BALB/c progenitors, when mated with DDK females. Our results indicate that a single locus, Om, is responsible for the DDK syndrome and is located on Chromosome (Chr) 11, very close to the Sigje locus.
DDK近交系小鼠有一个显著特点：当DDK雌鼠与其他品系的雄鼠杂交时，它们的生育力会降低，而反交（非DDK雌鼠×DDK雄鼠）则具有生育力（若杉等人，1967年；若杉，1973年）。低生育力是由早期胚胎致死所致，F1胚胎在桑椹胚晚期至囊胚早期死亡。遗传分析（若杉，1974年）以及核与细胞质移植（勒纳尔和巴比内，1986年；巴比内等人，1990年；曼，1986年）表明，胚胎发育失败是由于DDK母体编码的细胞质产物与非DDK父本基因组之间不兼容。为了阐明这种胚胎致死的遗传决定因素，我们分析了回交BALB/c雌鼠×（BALB/c×DDK）F1雄鼠的雄性后代的生育力，以及一组由DDK和BALB/c祖代建立的重组近交（RI）品系的雄鼠与DDK雌鼠交配后的生育力。我们的结果表明，一个单一基因座Om与DDK综合征有关，它位于第11号染色体上，非常靠近Sigje基因座。
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