LIU C, EATON M D, HEYL J T
J Exp Med. 1959 Jun 1;109(6):545-56. doi: 10.1084/jem.109.6.545.
By using the indirect method of fluorescent staining to study the antibody response in patients with primary atypical pneumonai associated with the development of cold agglutinin, it was found that the PAP antibody developed during the 2nd and 3rd week of the illness, and persisted for over a year, and is not related to the cold and streptococcus MG agglutinins. The development of the PAP fluorescent staining antibody paralleled the neutralizing antibody for the PAP virus as tested in cotton rats. The sensitivity of this specific serological test was indicated by the observation that 67 to 92 per cent of the patients in several outbreaks of PAP showed a rise of antibody titer during convalescence. Absorption of the sera with various tissue powders did not affect the PAP antibody detected by this method.
通过使用间接荧光染色法研究原发性非典型肺炎患者中与冷凝集素产生相关的抗体反应,发现肺炎支原体抗体在疾病的第2周和第3周出现,并持续一年以上,且与冷凝集素和链球菌MG凝集素无关。肺炎支原体荧光染色抗体的产生与棉鼠体内检测到的肺炎支原体病毒中和抗体平行。在几次肺炎支原体感染暴发中,67%至92%的患者在恢复期抗体滴度升高,这一观察结果表明了这种特异性血清学检测的敏感性。用各种组织粉末吸收血清并不影响用该方法检测到的肺炎支原体抗体。