Nishino T, Kiyohara H, Yamada H, Nagumo T
Department of Biophysics, School of Hygienic Sciences, Kitasato University, Kanagawa, Japan.
Phytochemistry. 1991;30(2):535-9. doi: 10.1016/0031-9422(91)83722-w.
The structure of a alpha-L-fucose-rich, sulphated polysaccharide (C-I) with a potent anticoagulant activity, which was isolated from the brown seaweed Ecklonia kurome, has been studied. Methylation analysis showed that C-I consisted mainly of 3-linked and 3,4-disubstituted fucopyranosyl residues in addition to non-reducing terminal fucofuranosyl and fucopyranosyl residues, 2,3-di- and 2,3,4-tri-substituted fucopyranosyl residues and galactopyranosyl residues with various glycosidic linkages. Methanolysis of C-I gave neutral di-, tri-, tetra- and highly polymerized-oligosaccharide fractions. GC-MS and methylation analysis indicated that di- and trisaccharide fractions consisted mainly of Fuc-(1----3)-Fuc and Fuc-(1----3)-Fuc-(1----3)-Fuc, respectively, in addition to small amounts of Fuc-(1----4)-Fuc, Fuc-(1----4)-Gal and Fuc-(1----3)-[Fuc-(1----2)-]Fuc. When methylated C-I was subjected to methanolysis for desulphation followed by remethylation with deuterated methyl iodide, most of deuteriomethyl groups substituted to position 4 of 3-linked Fuc.
对从褐藻黑昆布中分离得到的具有强抗凝活性的富含α-L-岩藻糖的硫酸化多糖（C-I）的结构进行了研究。甲基化分析表明，C-I除了含有非还原端的呋喃岩藻糖基和吡喃岩藻糖基残基、2,3-二取代和2,3,4-三取代的吡喃岩藻糖基残基以及具有各种糖苷键的吡喃半乳糖基残基外，主要由3-连接的和3,4-二取代的吡喃岩藻糖基残基组成。C-I的甲醇解得到中性二糖、三糖、四糖和高度聚合的寡糖馏分。气相色谱-质谱联用（GC-MS）和甲基化分析表明，二糖馏分和三糖馏分分别主要由Fuc-(1→3)-Fuc和Fuc-(1→3)-Fuc-(1→3)-Fuc组成，此外还含有少量的Fuc-(1→4)-Fuc、Fuc-(1→4)-Gal和Fuc-(1→3)-[Fuc-(1→2)-]Fuc。当甲基化的C-I进行甲醇解以脱硫，然后用氘代碘甲烷进行再甲基化时，大多数氘代甲基取代到3-连接的Fuc的4位。
