一种使用吗啉醇（1-脱氧野尻霉素）酶促合成高纯度麦芽糖的新颖高效方法。

A novel and efficient method for enzymatic synthesis of high purity maltose using moranoline (1-deoxynojirimycin).

作者信息

Maruo S, Yamashita H, Miyazaki K, Yamamoto H, Kyotani Y, Ogawa H, Kojima M, Ezure Y

机构信息

Chemistry Laboratories, Nippon Shinyaku Co., Ltd., Kyoto, Japan.

出版信息

Biosci Biotechnol Biochem. 1992 Sep;56(9):1406-9. doi: 10.1271/bbb.56.1406.

DOI:10.1271/bbb.56.1406

PMID:1368945

Abstract

A transglycosylation reaction with moranoline (1-deoxynojirimycin) was done with soluble starch as the glucosyl donor and Bacillus macerans amylase as a cyclodextrin glycosyltransferase [EC 2.4.1.19]. The resultant transglycosylation products with moranoline, obtained by treating the reaction mixture with a strong cation exchange resin, were hydrolyzed by beta-amylase [EC 3.2.1.2] from sweet potatoes. The hydrolysate was treated with a strong cation exchange resin, and high purity maltose was obtained.

摘要

以可溶性淀粉作为葡萄糖基供体，以浸麻芽孢杆菌淀粉酶作为环糊精糖基转移酶[EC 2.4.1.19]，与吗啉醇（1-脱氧野尻霉素）进行转糖基化反应。通过用强阳离子交换树脂处理反应混合物得到的与吗啉醇的转糖基化产物，用甘薯中的β-淀粉酶[EC 3.2.1.2]进行水解。水解产物用强阳离子交换树脂处理，得到高纯度麦芽糖。