二溴乙烷：小鼠显性致死试验和电泳特异性位点试验结果为阴性。

Ethylene dibromide: negative results with the mouse dominant lethal assay and the electrophoretic specific-locus test.

作者信息

Barnett L B, Lovell D P, Felton C F, Gibson B J, Cobb R R, Sharpe D S, Shelby M D, Lewis S E

机构信息

Research Triangle Institute, Research Triangle Park, NC 27709-2194.

出版信息

Mutat Res. 1992 Jun;282(2):127-33. doi: 10.1016/0165-7992(92)90085-v.

DOI:10.1016/0165-7992(92)90085-v

PMID:1377351

Abstract

Ethylene dibromide (1,2-dibromoethane; EDB) was tested for the induction of dominant lethal and electrophoretically-detectable specific-locus mutations in the germ cells of DBA/2J male mice. Males were treated with a single intraperitoneal injection of 100 mg/kg EDB and mated to two C57BL/6J females. In the dominant lethal assay, matings were carried out to measure the effect of EDB on meiotic and postmeiotic stages; germ cells representing spermatogonial stem cells were analyzed in the electrophoretic specific-locus test. Neither of these germ cell tests produced any evidence that EDB is a germ cell mutagen. It appears from these data and those reported in the literature that EDB, a genotoxic carcinogen that affects male fertility in some mammalian species, is not mutagenic in the germ cells of the male mouse.

摘要

对二溴乙烷（1,2 - 二溴乙烷；EDB）进行了测试，以检测其对DBA/2J雄性小鼠生殖细胞中显性致死和电泳可检测的特定基因座突变的诱导作用。雄性小鼠经腹腔注射100 mg/kg EDB单次处理后，与两只C57BL/6J雌性小鼠交配。在显性致死试验中，进行交配以测量EDB对减数分裂和减数分裂后阶段的影响；在电泳特定基因座试验中分析代表精原干细胞的生殖细胞。这两种生殖细胞试验均未产生任何证据表明EDB是一种生殖细胞诱变剂。从这些数据以及文献报道的数据来看，EDB作为一种影响某些哺乳动物物种雄性生育能力的遗传毒性致癌物，在雄性小鼠的生殖细胞中不具有致突变性。