Arfman N, Dijkhuizen L, Kirchhof G, Ludwig W, Schleifer K H, Bulygina E S, Chumakov K M, Govorukhina N I, Trotsenko Y A, White D
Department of Microbiology, University of Groningen, Haren, The Netherlands.
Int J Syst Bacteriol. 1992 Jul;42(3):439-45. doi: 10.1099/00207713-42-3-439.
The generic position of 14 strains of gram-positive bacteria able to use methanol as a growth substrate was determined. All are obligately aerobic, thermotolerant organisms that are able to grow at temperatures of 35 to 60 degrees C. Nine of the strains produce oval spores at a subterminal-to-central position in slightly swollen rod-shaped cells. DNA-DNA hybridization studies, 5S rRNA sequence analysis, and physiological characteristics revealed that all 14 strains cluster as a well-defined group and form a distinct new genospecies. Analysis of the 16S and 5S rRNA sequences indicated that this new species is distinct from Bacillus brevis but closely related to B. firmus and B. azotoformans. The name proposed for this new species is B. methanolicus. The type strain, PB1, has been deposited in the National Collection of Industrial and Marine Bacteria as NCIMB 13113.
确定了14株能够利用甲醇作为生长底物的革兰氏阳性菌的分类地位。所有菌株均为专性需氧、耐热微生物,能够在35至60摄氏度的温度下生长。其中9株菌株在略呈肿胀的杆状细胞中,于亚末端至中央位置产生椭圆形孢子。DNA-DNA杂交研究、5S rRNA序列分析和生理特征表明,所有14株菌株聚为一个界限分明的类群,形成一个独特的新基因型种。16S和5S rRNA序列分析表明,这个新物种与短短芽孢杆菌不同,但与坚强芽孢杆菌和固氮芽孢杆菌密切相关。为这个新物种提议的名称是甲醇芽孢杆菌。模式菌株PB1已作为NCIMB 13113保藏于国家工业和海洋细菌保藏中心。