COHEN H, VAN RAMSHORST J, TASMAN A
Bull World Health Organ. 1959;20(6):1133-50.
The use of mice for the assay of tetanus toxoids would offer considerable advantages over the use of guinea-pigs, but mice cannot readily be immunized with the fluid tetanus toxoid at present designated as the International Standard. This study shows, however, that the mouse is a very suitable laboratory animal for the comparison of adsorbed tetanus toxoids, and that an AlPO(4)-adsorbed vaccine, which is stable at 4 degrees C, is a satisfactory reference preparation. The log-dose-response lines of toxoids adsorbed on different quantities of AlPO(4) and on various quantities of another adsorbent ran parallel to those of the reference vaccine. The 95% confidence limits for the potencies of tetanus vaccines, diphtheria-tetanus vaccines, and diphtheria-pertussis-tetanus vaccines, determined by assay against the reference vaccine in mice, showed a high degree of reproducibility of the results.
使用小鼠来测定破伤风类毒素比使用豚鼠具有相当多的优势,但目前指定为国际标准的液体破伤风类毒素难以使小鼠轻易免疫。然而,这项研究表明,小鼠是用于比较吸附破伤风类毒素的非常合适的实验动物,并且一种在4℃稳定的磷酸铝吸附疫苗是一种令人满意的参考制剂。吸附在不同量磷酸铝和不同量的另一种吸附剂上的类毒素的对数剂量反应线与参考疫苗的反应线平行。通过在小鼠中与参考疫苗进行测定来确定的破伤风疫苗、白喉 - 破伤风疫苗和白喉 - 百日咳 - 破伤风疫苗效力的95%置信限显示出结果具有高度的可重复性。