哈蒙德氏巴贝斯虫和刚地弓形虫子孢子的同工酶分析。

Isoenzyme analysis of Hammondia hammondi and Toxoplasma gondii sporozoites.

作者信息

Dardé M L, Riahi H, Bouteille B, Pestre-Alexandre M

机构信息

Service de Parasitologie-Mycologie, CHU Dupuytren, Limoges, France.

出版信息

J Parasitol. 1992 Aug;78(4):731-4.

PMID:1386109

Abstract

Isoenzyme analysis using isoelectrofocusing in polyacrylamide gels was used to distinguish Hammondia hammondi and Toxoplasma gondii sporozoites. Five enzyme systems were studied: aconitase (EC 4.2.1.3), aspartate aminotransferase (EC 2.6.1.1), glucose phosphate isomerase (EC 5.3.1.9), lactate dehydrogenase (EC 1.1.1.27), and phosphoglucomutase (EC 2.7.5.1). Three stocks of T. gondii belonging to 3 zymodemes were compared to 1 stock of H. hammondi. Hammondia hammondi differed from T. gondii at all 5 loci analyzed. This was observed for all 3 zymodemes of T. gondii. These results indicated clear genetic differences between the 2 species.

摘要

采用聚丙烯酰胺凝胶等电聚焦法进行同工酶分析，以区分哈蒙德氏哈蒙德虫和刚地弓形虫的子孢子。研究了五个酶系统：乌头酸酶（EC 4.2.1.3）、天冬氨酸转氨酶（EC 2.6.1.1）、葡萄糖磷酸异构酶（EC 5.3.1.9）、乳酸脱氢酶（EC 1.1.1.27）和磷酸葡萄糖变位酶（EC 2.7.5.1）。将属于3个酶型的3株刚地弓形虫与1株哈蒙德氏哈蒙德虫进行比较。在分析的所有5个基因座上，哈蒙德氏哈蒙德虫与刚地弓形虫不同。在刚地弓形虫的所有3个酶型中均观察到这一点。这些结果表明这两个物种之间存在明显的遗传差异。