ANDRES G A, MORGAN C, HSU K C, RIFKIND R A, SEEGAL B C
J Exp Med. 1962 May 1;115(5):929-36. doi: 10.1084/jem.115.5.929.
Ferritin-conjugated antibody has been used to identify by electron microscopy the sites at which nephrotoxic globulins localize in rat kidney during acute experimental glomerulonephritis. Antibody was concentrated in the glomerular basement membrane and in basement membrane-like material contained in distended cisternae of the endoplasmic reticulum. These data confirm and amplify, at the ultrastructural level, the results of studies obtained with the fluorescent antibody technique, and are consistent with the hypothesis that the cisternae and capillary basement membrane possess common proteins.
铁蛋白结合抗体已被用于通过电子显微镜鉴定在急性实验性肾小球肾炎期间肾毒性球蛋白在大鼠肾脏中定位的部位。抗体集中在内质网扩张池所含的肾小球基底膜和类基底膜物质中。这些数据在超微结构水平上证实并扩展了用荧光抗体技术获得的研究结果,并且与内质网池和毛细血管基底膜具有共同蛋白质的假说一致。