双特异性抗人红细胞恒河猴-D抗原x抗FcγRI靶向抗体依赖的单核白细胞介导的细胞毒性和吞噬作用。

Bispecific anti-human red blood Rhesus-D antigen x anti Fc gamma RI targeted antibody-dependent cell-mediated cytotoxicity and phagocytosis by mononuclear leucocytes.

作者信息

Deramoudt E X, Gilard C, Lepine N, Alonso J M, Romet-Lemonne J L

机构信息

Fondation Nationale De Transfusion Sanguine, Les Ulis, France.

出版信息

Clin Exp Immunol. 1992 Aug;89(2):310-4. doi: 10.1111/j.1365-2249.1992.tb06951.x.

DOI:10.1111/j.1365-2249.1992.tb06951.x

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1554448/

Abstract

The Fc receptor mediated antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis induced by bispecific antibody (BsAb) to the high-affinity Fc receptor for IgG (Fc gamma RI) and to human red blood group antigen RhD were studied in vitro, using human mononuclear leucocytes as effector cells. The results were compared with those obtained by using a human monoclonal IgG1 anti-RhD used alone and a reference human polyclonal anti-RhD antibody. The effect of non-specific human IgG on FcR-mediated functions by mononuclear leucocytes was checked. The results demonstrate that BsAb presents a high resistance of Fc-mediated function to blockade by non-specific human IgG compared with that of both polyclonal and monoclonal anti-RhD antibodies. These results further encourage possible clinical application of bispecific antibody in passive immunotherapy.

摘要

使用人单核白细胞作为效应细胞，在体外研究了双特异性抗体（BsAb）对IgG高亲和力Fc受体（FcγRI）和人红细胞血型抗原RhD介导的Fc受体依赖性抗体介导的细胞毒性（ADCC）和吞噬作用。将结果与单独使用人单克隆IgG1抗RhD和参考人多克隆抗RhD抗体获得的结果进行比较。检测了非特异性人IgG对单核白细胞FcR介导功能的影响。结果表明，与多克隆和单克隆抗RhD抗体相比，BsAb的Fc介导功能对非特异性人IgG的阻断具有高抗性。这些结果进一步推动了双特异性抗体在被动免疫治疗中可能的临床应用。

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本文引用的文献

1

Production of target-specific effector cells using hetero-cross-linked aggregates containing anti-target cell and anti-Fc gamma receptor antibodies.

J Exp Med. 1984 Dec 1;160(6):1686-701. doi: 10.1084/jem.160.6.1686.

2

Recombinant immune interferon increases immunoglobulin G Fc receptors on cultured human mononuclear phagocytes.

J Clin Invest. 1983 Jul;72(1):393-7. doi: 10.1172/jci110980.

3

A mew method for detection of red blood cell antibodies.

Transfusion. 1968 Nov-Dec;8(6):372-80. doi: 10.1111/j.1537-2995.1968.tb02439.x.

4

Measurement of antibody-dependent cell-mediated cytotoxicity by Hb-enzyme release assay (Hb-ERA).

J Immunol Methods. 1985 Dec 27;85(2):325-33. doi: 10.1016/0022-1759(85)90141-3.

5

Anti-ovarian carcinoma anti-T3 heteroconjugates or hybrid antibodies induce tumor cell lysis by cytotoxic T-cells.

Int J Cancer Suppl. 1988;2:18-21. doi: 10.1002/ijc.2910410707.

6

The use of macrophages stimulated by immune interferon as indicator cells in the mononuclear phagocyte assay.

Clin Lab Haematol. 1987;9(4):399-408. doi: 10.1111/j.1365-2257.1987.tb00578.x.

7

Antibody dependent cell mediated cytotoxicity against anti-D sensitized human erythrocytes. Influence of various effector cells and enzyme treatment of the target cells.

APMIS. 1988 Mar;96(3):250-6.

8

Human monoclonal antibody against Rh(D) antigen: partial characterization of the Rh(D) polypeptide from human erythrocytes.

Blood. 1987 May;69(5):1491-7.

9

Monoclonal antibodies to Fc receptors for IgG on human mononuclear phagocytes. Antibody characterization and induction of superoxide production in a monocyte cell line.

J Biol Chem. 1986 Sep 25;261(27):12856-64.

10

Lysis of cells infected with HIV-1 by human lymphocytes targeted with monoclonal antibody heteroconjugates.

J Immunol. 1988 Apr 15;140(8):2609-13.

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