ISOLATION OF THE MONKEY BRAIN: IN VITRO PREPARATION AND MAINTENANCE.

Sustained viability of the primate brain, as a totally isolated organ preparation, was achieved by utilizing an extracorporeal (compatible donor) circulation. Five rhesus monkey brains, completely isolated neurogenically and vascularly, were perfused in vitro for 30 to 180 minutes. Retention of biological activity was evidenced by: (i) persistent electrocortical activity, and (ii) significant mean A-Vo(o2) (5.8 volumes percent) and V-Aco(co2) (5.0 volumes percent) differences across the isolated brain.