SIROTNAK F M, LUNT R B, HUTCHISON D J
J Bacteriol. 1963 Oct;86(4):735-9. doi: 10.1128/jb.86.4.735-739.1963.
Sirotnak, Francis M. (Sloan-Kettering Institute for Cancer Research, New York, N.Y.), Ramona B. Lunt, and Dorris J. Hutchison. Alteration in transformability of Diplococcus pneumoniae after the acquisition of genetic determinants inducing resistance to erythromycin. J. Bacteriol. 86:735-739. 1963.-The genetic alteration of a highly transformable (competent) recipient strain of Diplococcus pneumoniae by the transformation of at least two of three identified erythromycin-resistance determinants (Ery(a), Ery(b), Ery(c)) results in a marked decrease (Ery(a-b) recombinant) or complete loss (Ery(a-c) recombinant) in the ability to be transformed. The occurrence of transformable cells in cultures of the R6 Ery(a-b) recombinant strains, although greatly diminished, still varies during growth in a manner characteristic of the fully competent parent strain. Both Ery(b) and Ery(c) determinants appear to be linked to Ery(a). In experiments using P(32)-labeled deoxyribonucleic acid (DNA), data correlating DNA uptake with transformation show a decrease or loss in uptake capacity of the erythromycin-resistant strains.
西罗特纳克，弗朗西斯·M.（纽约市斯隆 - 凯特琳癌症研究所），雷蒙娜·B.伦特，多丽丝·J.哈钦森。肺炎双球菌获得诱导对红霉素耐药的遗传决定因素后转化能力的改变。《细菌学杂志》86:735 - 739。1963年。——通过转化三种已鉴定的红霉素耐药决定因素（Ery(a)、Ery(b)、Ery(c)）中的至少两种，对肺炎双球菌的一个高度可转化（感受态）受体菌株进行遗传改变，导致转化能力显著下降（Ery(a - b)重组体）或完全丧失（Ery(a - c)重组体）。R6 Ery(a - b)重组体菌株培养物中可转化细胞的出现，尽管大大减少，但在生长过程中仍以完全感受态亲代菌株的特征方式变化。Ery(b)和Ery(c)决定因素似乎都与Ery(a)连锁。在使用P(32)标记的脱氧核糖核酸（DNA）的实验中，将DNA摄取与转化相关的数据表明，红霉素耐药菌株的摄取能力下降或丧失。
