N-乙基马来酰亚胺对鲎血细胞凝集的抑制作用。

N-ETHYLMALEIMIDE INHIBITION OF HORSESHOE CRAB HEMOCYTE AGGLUTINATION.

作者信息

BRYAN F T, ROBINSON C W, GILBERT C G, LANGDELL R D

出版信息

Science. 1964 May 29;144(3622):1147-8. doi: 10.1126/science.144.3622.1147.

DOI:10.1126/science.144.3622.1147

Abstract

The sulfhydryl inhibitor N-ethylmaleimide (NEM) inhibits the agglutination of horseshoe crab hemocytes. The inhibitory action is neutralized by adding cysteine to the NEM before it is mixed with the hemolymph. This new method for arresting horseshoe crab hemocyte agglutination suggests the participation of sulfhydryl groups in the process.

摘要

巯基抑制剂N-乙基马来酰亚胺（NEM）可抑制鲎血细胞的凝集。在将NEM与血淋巴混合之前，加入半胱氨酸可中和其抑制作用。这种阻止鲎血细胞凝集的新方法表明巯基参与了这一过程。