来自抗生链霉菌的多纤维素酶系统。
MULTIPLE CELLULASE SYSTEM FROM STREPTOMYCES ANTIBIOTICUS.
作者信息
ENGER M D, SLEEPER B P
出版信息
J Bacteriol. 1965 Jan;89(1):23-7. doi: 10.1128/jb.89.1.23-27.1965.
Enger, M. D. (North Dakota State University, Fargo), and B. P. Sleeper. Multiple cellulase system from Streptomyces antibioticus. J. Bacteriol. 89:23-27. 1965.-Starch-block zone electrophoresis was used to isolate five electrophoretically distinct, active cellulolytic components (I to V) from the crude extracellular cellulase system of Streptomyces antibioticus (strain C2A). Agar diffusion precipitin analyses demonstrated the immunological identity of components I, II, and III, and the nonidentity of IV and V with each other and with I to III. Kinetic studies of the purified enzymes showed a sharp decrease in the viscosity of the substrate, carboxymethylcellulose, with only a small increase in reducing sugars. These results indicated that all five enzymes are endocellulases.
恩格，M. D.（北达科他州立大学，法戈）和B. P. 斯利珀。抗生链霉菌的多种纤维素酶系统。《细菌学杂志》89:23 - 27。1965年。——采用淀粉区带电泳从抗生链霉菌（菌株C2A）的粗细胞外纤维素酶系统中分离出五个电泳性质不同的活性纤维素分解组分（I至V）。琼脂扩散沉淀素分析表明组分I、II和III具有免疫同一性，而IV和V彼此之间以及与I至III均不相同。对纯化酶的动力学研究表明，底物羧甲基纤维素的粘度急剧下降，而还原糖仅有少量增加。这些结果表明所有这五种酶都是内切纤维素酶。
Zotero 插件