海鞘卵母细胞中细胞质碱性蛋白的细胞化学研究
A CYTOCHEMICAL STUDY OF CYTOPLASMIC BASIC PROTEINS IN THE ASCIDIAN OOCYTE.
作者信息
DAVENPORT R, DAVENPORT J C
出版信息
J Cell Biol. 1965 May;25(2):319-26. doi: 10.1083/jcb.25.2.319.
Abstract
The cytoplasm of young oocytes of the ascidians contains high concentrations of proteins which are stainable with alkaline fast green at pH 8.1 and above. These proteins cannot be stained even with acid dyes at low pH unless RNA is removed. Deamination and formalin blockage of amino groups is incapable of destroying the net positive charge on these protein molecules in the presence of RNA, but these treatments destroy the charge if RNA is removed. It is therefore concluded that basic proteins and RNA exist as a nucleoprotein complex in the ribosomes of these young oocytes. The detectable RNA of the mature oocytes and unfertilized eggs shows no evidence of being associated with basic proteins.
摘要
海鞘幼龄卵母细胞的细胞质中含有高浓度的蛋白质，这些蛋白质在pH 8.1及以上时可用碱性固绿染色。除非去除RNA，否则即使在低pH值下用酸性染料也无法对这些蛋白质进行染色。在有RNA存在的情况下，氨基的脱氨作用和福尔马林封闭不能破坏这些蛋白质分子上的净正电荷，但如果去除RNA，这些处理会破坏电荷。因此可以得出结论，在这些幼龄卵母细胞的核糖体中，碱性蛋白质和RNA以核蛋白复合物的形式存在。成熟卵母细胞和未受精卵中可检测到的RNA没有显示出与碱性蛋白质相关的证据。
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