一种用于测定人血浆中游离11-羟基皮质类固醇的简易荧光法。
A simple fluorimetric method for the estimation of free 11-hydroxycorticoids in human plasma.
作者信息
MATTINGLY D
出版信息
J Clin Pathol. 1962 Jul;15(4):374-9. doi: 10.1136/jcp.15.4.374.
Abstract
A simple fluorimetric method is described for measuring free 11-hydroxycorticoids in human plasma. Only 2 ml. of plasma is required for each estimation and the fluorescence is read in a standard direct reading fluorimeter. Six estimations can be completed in one and a half hours. It thus compares favourably with the methods in current use for estimating urinary steroids, and has the added advantage of not being dependent on the accurate collection of 24-hour urine samples.
摘要
本文描述了一种用于测定人血浆中游离11-羟基皮质类固醇的简单荧光法。每次测定仅需2毫升血浆，荧光在标准直读荧光计中读取。一个半小时内可完成六次测定。因此，它与目前用于测定尿类固醇的方法相比具有优势，并且还有不依赖于准确收集24小时尿样的额外优点。
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