Kweon Chang Hee, Kwon Byung Joon, Ko Young Joon, Kenichi Sakamoto
Virology Division, National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, 480 Anyang 6-dong, Anyang, Gyeong Gi Do, South Korea.
J Virol Methods. 2003 Oct;113(1):13-8. doi: 10.1016/s0166-0934(03)00217-9.
VP7, the sero-group common antigen, of African horsesickness virus (AHSV-4) was expressed in insect cells by recombinant baculovirus. To develop a specific diagnostic method, monoclonal antibody (Mab) against VP7 was prepared and investigated as diagnostic reagent with the baculovirus expressed VP7. However, the Mab against VP7 of AHSV cross-reacted with Chuzan virus by the indirect immunofluorescence assay (IFA), confirming the presence of conserved domain of VP7 among Orbiviruses. This study describes two types of ELISA; Mab linked indirect (I-ELISA) and competitive-ELISA (C-ELISA) using baculovirus expressed VP7 as an antigen. These ELISAs were compared for serodiagnosis of AHSV showing that C-ELISA was more specific than I-ELISA. The results indicated that C-ELISA is applicable to serodiagnosis of AHSV regardless of serotypes.
非洲马瘟病毒（AHSV-4）的血清群共同抗原VP7通过重组杆状病毒在昆虫细胞中表达。为开发一种特异性诊断方法，制备了抗VP7的单克隆抗体（Mab），并将其作为诊断试剂与杆状病毒表达的VP7一起进行研究。然而，通过间接免疫荧光试验（IFA），抗AHSV VP7的Mab与楚山病毒发生交叉反应，证实了环状病毒中VP7保守结构域的存在。本研究描述了两种类型的酶联免疫吸附测定（ELISA）；以杆状病毒表达的VP7作为抗原的Mab连接间接ELISA（I-ELISA）和竞争ELISA（C-ELISA）。对这些ELISA进行比较以用于AHSV的血清学诊断，结果表明C-ELISA比I-ELISA更具特异性。结果表明，C-ELISA适用于AHSV的血清学诊断，而与血清型无关。
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