在牛白血病血清学诊断中PLA与AGID及ELISA结果的比较

Comparison of PLA with AGID and ELISA results in serology diagnosis of bovine leukosis.

作者信息

Buzała E, Dereń W

机构信息

Department of Cell Pathology, National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland.

出版信息

Pol J Vet Sci. 2003;6(3 Suppl):9-11.

PMID:14509350

Abstract

The aim of this study was to assess the diagnostic value of Peroxidase Linked Assay (PLA) for detecting the bovine leukemia virus (BLV) infection. Two negative and two positive reference sera were applied to 204 serum samples from dairy cows from 3 different farms (I, II and III). The best results of serological testing were obtained in the herd infected with BLV for several years (I). Amongst 10 serum samples taken at random, all were positive in AGID, ELISA and PLA. Serological tests of samples from group II revealed: 34 (73.9%) positive results in AGID and 39 (84.7%) either in ELISA or PLA. In group III positive results in AGID occured in 81.8% of cases, and 91.9% and 92.9% in ELISA and PLA respectively. The mean OD value for positive samples in groups II and III ranged between 0.943 and 0.215. Immunoperoxidase PLA is a specific and sensitive test for detection of BLV infections.

摘要

本研究的目的是评估过氧化物酶联检测法（PLA）对检测牛白血病病毒（BLV）感染的诊断价值。将两份阴性和两份阳性参考血清应用于来自3个不同农场（I、II和III）的204份奶牛血清样本。在感染BLV数年的牛群（I）中获得了最佳血清学检测结果。在随机抽取的10份血清样本中，所有样本在琼脂凝胶免疫扩散试验（AGID）、酶联免疫吸附测定（ELISA）和PLA中均呈阳性。来自II组样本的血清学检测显示：AGID中有34份（73.9%）呈阳性结果，ELISA或PLA中有39份（84.7%）呈阳性结果。在III组中，AGID的阳性率为81.8%，ELISA和PLA的阳性率分别为91.9%和92.9%。II组和III组阳性样本的平均光密度（OD）值在0.943至0.215之间。免疫过氧化物酶PLA是检测BLV感染的一种特异性和敏感性均较高的检测方法。