“热毒清”对淋巴细胞活化、增殖及膜流动性的影响。

Effect of "re du qing" on the activation, proliferation and membrane fluidity of lymphocytes.

作者信息

Zhang G M, Hao T L, Yang Y Z, Feng Z H, Jiang Z Y, Zhou B, Zhang H

机构信息

Department of Molecular Biology, Tongji Medical University, Wuhan.

出版信息

J Tongji Med Univ. 1992;12(3):150-3. doi: 10.1007/BF02887815.

DOI:10.1007/BF02887815

PMID:1453504

Abstract

Effects of Chinese Medicinal Preparation "Re Du Qing" (RDQ) on the activation, proliferation and membrane fluidity of T lymphocytes from human peripheral blood were studied by means of 3H-TdR incorporation and DPH fluorescence polarization. The results showed that "RDQ" can: 1) significantly inhibit the activation of T lymphocytes; 2) restrain the proliferation of activated T lymphoblasts in the presence of exogenous interleukin-2 (IL-2); and 3) increase the membrane fluidity of T lymphocytes and antagonize the decreased fluidity of lymphocyte membrane mediated by Con A or PHA. The functional abnormalities of T lymphocytes in some autoimmune diseases such as arthritis and the usefulness of RDQ in the treatment of these diseases were also discussed.

摘要

采用³H-TdR掺入法及DPH荧光偏振法，研究了中药制剂“热毒清”（RDQ）对人外周血T淋巴细胞活化、增殖及膜流动性的影响。结果表明，“热毒清”能：1）显著抑制T淋巴细胞的活化；2）在有外源性白细胞介素-2（IL-2）存在的情况下，抑制活化的T淋巴母细胞的增殖；3）增加T淋巴细胞的膜流动性，并对抗由刀豆蛋白A（Con A）或植物血凝素（PHA）介导的淋巴细胞膜流动性降低。文中还讨论了某些自身免疫性疾病如关节炎中T淋巴细胞的功能异常以及“热毒清”在治疗这些疾病中的作用。