Ward O P, Moo-Young M
Institute for Biotechnology Research, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1.
Biotechnol Adv. 1988;6(1):39-69. doi: 10.1016/0734-9750(88)90573-3.
In general, enzyme thermostability is an intrinsic property, determined by the primary structure of the protein. However, external environmental factors including cations, substrates, co-enzymes, modulators, polyols and proteins often increase enzyme thermostability. With some exceptions, enzymes present in thermophiles are more stable than their mesophilic counterparts. Some organisms produce enzymes with different thermal stability properties when grown at lower and higher temperatures. There are commercial advantages in carrying out enzymic reactions at higher temperatures. Some industrial enzymes exhibit high thermostability. More stable forms of other industrial enzymes are eagerly being sought.
一般来说,酶的热稳定性是一种内在特性,由蛋白质的一级结构决定。然而,包括阳离子、底物、辅酶、调节剂、多元醇和蛋白质在内的外部环境因素常常会提高酶的热稳定性。除了一些例外情况,嗜热菌中存在的酶比它们的中温对应物更稳定。一些生物体在较低和较高温度下生长时会产生具有不同热稳定性的酶。在较高温度下进行酶促反应具有商业优势。一些工业酶表现出高的热稳定性。人们正急切地寻找其他工业酶的更稳定形式。