B细胞慢性淋巴细胞白血病中T细胞产生细胞内白细胞介素-2、肿瘤坏死因子-α和干扰素-γ 。

Production of intracellular IL-2, TNF-alpha, and IFN-gamma by T cells in B-CLL.

作者信息

Gallego A, Vargas J A, Castejón R, Citores M J, Romero Y, Millán I, Durántez A

机构信息

Service of Internal Medicine I, Hospital Universitario Puerta de Hierro, Universidad Autónoma, Madrid, Spain.

出版信息

Cytometry B Clin Cytom. 2003 Nov;56(1):23-9. doi: 10.1002/cyto.b.10052.

DOI:10.1002/cyto.b.10052

PMID:14582134

Abstract

BACKGROUND

Recent evidence indicates that the slowly expanding population of CD5(+) B cells that characterizes B-cell chronic lymphocytic leukemia (B-CLL) could be related to defects in the response to cytokine produced by T cells that regulate apoptosis. We studied the intracellular expressions of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma) in T-helper 1 cells (Th1 response) of B-CLL.

METHODS

Peripheral blood mononuclear cells from 21 healthy individuals and purified T cells from 21 early-stage and 15 late-stage B-CLL patients were activated with phorbol myristate acetate and ionomycin. The Th1 cytoplasmic cytokines were evaluated in CD4(+) and CD8(+) T cells by flow cytometry.

RESULTS

The percentages of CD4(+) and CD8(+) T cells positive for IL-2 were significantly lower in B-CLL patients than in healthy individuals (P = 0.030 and 0.049, respectively). No significant differences in TNF-alpha or IFN-gamma intracellular expressions were found between patients and healthy individuals. TNF-alpha- and IFN-gamma-expressing CD8 T cells were disease stage dependent, being significantly higher in late-stage patients (P < 0.001 for both cytokines).

CONCLUSIONS

Our present observations suggested that Th1 cytokines may be of major importance in the pathogenesis of B-CLL.

摘要

背景

最近有证据表明，表征B细胞慢性淋巴细胞白血病（B-CLL）的缓慢扩增的CD5(+) B细胞群体可能与调节细胞凋亡的T细胞产生的细胞因子应答缺陷有关。我们研究了B-CLL患者辅助性T1细胞（Th1应答）中白细胞介素-2（IL-2）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）的细胞内表达。

方法

用佛波酯肉豆蔻酸酯和离子霉素激活21名健康个体的外周血单个核细胞以及21例早期和15例晚期B-CLL患者的纯化T细胞。通过流式细胞术评估CD4(+)和CD8(+) T细胞中的Th1细胞质细胞因子。

结果

B-CLL患者中IL-2阳性的CD4(+)和CD8(+) T细胞百分比显著低于健康个体（分别为P = 0.030和0.049）。患者与健康个体之间在TNF-α或IFN-γ细胞内表达上未发现显著差异。表达TNF-α和IFN-γ的CD8 T细胞与疾病分期相关，在晚期患者中显著更高（两种细胞因子均P < 0.001）。