[人羊膜FL细胞对低浓度N-甲基-N'-硝基-N-亚硝基胍细胞反应的蛋白质组学分析]
[Proteomic analysis of cellular responses to low concentration of N-methyl-N'-nitro-N-nitrosoguanidine in human amnion FL cells].
作者信息
Jin Jing-hua, Gao Zhi-hua, Yang Jun, Yu Ying-nian
机构信息
Department of Pathophysiology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2003 Oct;32(5):375-9. doi: 10.3785/j.issn.1008-9292.2003.05.002.
OBJECTIVE
To investigate the protein profile after treatment of low concentration of N- methyl-N'-nitro-N-nitrosoguanidine (MNNG) in human FL cells.
METHODS
After MNNG treatment, whole cellular proteins were separated using two-dimensional gel electrophoresis and visualized by silver staining; the digitized images were analyzed with 2D analysis software. The differentially expressed protein spots were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).
RESULT
More than 60 proteins showed significant changes in MNNG-treated cells compared to control cells (DMSO treatment). There were 18 protein spots detected only after MNNG treatment, while 13 protein spots were detected only in the control cells. Moreover, the levels of another 31 proteins were either increased or decreased in MNNG-treated FL cells. And some of the proteins were identified by MALDI-TOF-MS.
CONCLUSION
There are significant alterations of protein profile after MNNG attack.
目的
研究低浓度N-甲基-N'-硝基-N-亚硝基胍（MNNG）处理后人FL细胞的蛋白质谱。
方法
MNNG处理后，采用二维凝胶电泳分离全细胞蛋白质，银染显色；用二维分析软件分析数字化图像。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定差异表达的蛋白质斑点。
结果
与对照细胞（二甲基亚砜处理）相比，MNNG处理的细胞中有60多种蛋白质显示出显著变化。仅在MNNG处理后检测到18个蛋白质斑点，而仅在对照细胞中检测到13个蛋白质斑点。此外，MNNG处理的FL细胞中另外31种蛋白质的水平要么升高要么降低。其中一些蛋白质通过MALDI-TOF-MS进行了鉴定。
结论
MNNG攻击后蛋白质谱有显著改变。
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