I型糖尿病患者血清中针对牛胰岛素的IgG水平。

Serum IgG levels to bovine insulin in type I diabetes mellitus.

作者信息

Neyestani Tirang R, Djalali Mahmoud, Pezeshki Mohamad, Siassi Fereydoun, Eshraghian Mohamad R, Rajab Asadollah, Keshavarz Ali

机构信息

National Nutrition and Food Technology Research Institute, Shaheed Beheshti University, Tehran, Iran. neytr.yahoo.com

出版信息

Indian J Pediatr. 2003 Sep;70(9):701-5. doi: 10.1007/BF02724310.

DOI:10.1007/BF02724310

PMID:14620184

Abstract

OBJECTIVES

This study was undertaken to determine humoral immune response to bovine insulin in Iranian children with type 1 diabetes mellitus.

METHODS

Serum samples were taken from 93 children aged 4-17 years with type I diabetes mellitus from two centers in Iran (the Iranian Association of Diabetes in Tehran and Center for Diabetes Research in Hamedan), 17 apparently healthy siblings of the diabetic patients (related controls), 28 apparently healthy age- and sex- matched controls (unrelated controls), 14 patients aged 11-15 years with auto-immune thyroiditis, and 45 patients with type II diabetes (aged 44-68 years). Samples were then examined for specific IgG to bovine insulin by enzyme-linked immunosorbent assay (ELISA). A questionnaire on medical history, duration of exclusive and non-exclusive breast feeding and daily intake of dairy products was completed before bleeding.

RESULTS

Duration of exclusive and non-exclusive breast-feeding showed no significant difference between patients with type I diabetes, related and unrelated controls and thyroid patients. Diabetic children, however, had significantly higher serum levels of anti-bovine insulin IgG than did unrelated and related healthy controls and patients with type II diabetes (P< 0.01). There was no significant difference between healthy siblings of diabetic children and unrelated controls. In type I diabetic patients and their healthy siblings, serum levels of IgG to bovine insulin were inversely correlated with the duration of non-exclusive breast feeding (rs= -0.37, P= 0.016 and rs= -0.53, P= 0.049, respectively). There was no correlation between serum levels of IgG to bovine insulin with daily intake of dairy products. Bovine insulin cross-reacted with human insulin as judged by ELISA inhibition assay.

CONCLUSION

The emergence of anti-insulin antibodies in Iranian patients with type I DM, which is associated with the duration of breast-feeding is less likely to be due to early exposure of infants with the proteins found in cow's milk. One speculation could be that the production of antibodies to insulin in type I diabetes may just be a physiologic response (probably to increase the half-life of the circulating insulin). The importance of anti-insulin antibodies in type I diabetes mellitus needs further studies.

摘要

目的

本研究旨在确定伊朗1型糖尿病儿童对牛胰岛素的体液免疫反应。

方法

从伊朗两个中心（德黑兰的伊朗糖尿病协会和哈马丹糖尿病研究中心）的93名4 - 17岁1型糖尿病儿童、17名糖尿病患者的明显健康的同胞（相关对照）、28名年龄和性别匹配的明显健康对照（非相关对照）、14名11 - 15岁的自身免疫性甲状腺炎患者以及45名2型糖尿病患者（年龄44 - 68岁）采集血清样本。然后通过酶联免疫吸附测定（ELISA）检测样本中针对牛胰岛素的特异性IgG。在采血前完成一份关于病史、纯母乳喂养和非纯母乳喂养持续时间以及乳制品每日摄入量的问卷。

结果

纯母乳喂养和非纯母乳喂养的持续时间在1型糖尿病患者、相关和非相关对照以及甲状腺疾病患者之间没有显著差异。然而，糖尿病儿童的抗牛胰岛素IgG血清水平显著高于非相关和相关健康对照以及2型糖尿病患者（P < 0.01）。糖尿病儿童的健康同胞与非相关对照之间没有显著差异。在1型糖尿病患者及其健康同胞中，牛胰岛素IgG血清水平与非纯母乳喂养持续时间呈负相关（rs = -0.37，P = 0.016和rs = -0.53，P = 0.049）。牛胰岛素IgG血清水平与乳制品每日摄入量之间没有相关性。通过ELISA抑制试验判断，牛胰岛素与人胰岛素发生交叉反应。