中介体复合物与活跃基因增强子的关联。
Association of the Mediator complex with enhancers of active genes.
作者信息
Kuras Laurent, Borggrefe Tilman, Kornberg Roger D
机构信息
Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France.
出版信息
Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):13887-91. doi: 10.1073/pnas.2036346100. Epub 2003 Nov 17.
Abstract
The multiprotein Mediator complex has been shown to interact with gene-specific regulatory proteins and RNA polymerase II in vitro. Here, we use chromatin immunoprecipitation to analyze the recruitment of Mediator to GAL genes of yeast in vivo. We find that Mediator associates exclusively with transcriptionally active and not inactive GAL genes. This association maps to the upstream activating sequence, rather than the core promoter, and is independent of RNA polymerase II, general transcription factors, and core promoter sequences. These findings support the idea of Mediator as a primary conduit of regulatory information from enhancers to promoters in eukaryotic cells.
摘要
多蛋白中介体复合物已被证明在体外可与基因特异性调节蛋白及RNA聚合酶II相互作用。在此，我们利用染色质免疫沉淀法在体内分析中介体与酵母GAL基因的募集情况。我们发现中介体仅与转录活跃而非转录不活跃的GAL基因相关联。这种关联定位于上游激活序列，而非核心启动子，并且独立于RNA聚合酶II、通用转录因子及核心启动子序列。这些发现支持了中介体作为真核细胞中从增强子到启动子的调节信息主要传导途径的观点。
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本文引用的文献
1
Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly.全基因组转录和起始前复合物组装过程中酵母必需TAF的系统分析
EMBO J. 2003 Jul 1;22(13):3395-402. doi: 10.1093/emboj/cdg336.
2
Independent recruitment in vivo by Gal4 of two complexes required for transcription.Gal4在体内独立募集转录所需的两种复合物。
Mol Cell. 2003 May;11(5):1301-9. doi: 10.1016/s1097-2765(03)00144-8.
3
Structure of the yeast RNA polymerase II holoenzyme: Mediator conformation and polymerase interaction.酵母RNA聚合酶II全酶的结构：中介体构象与聚合酶相互作用
Mol Cell. 2002 Aug;10(2):409-15. doi: 10.1016/s1097-2765(02)00598-1.
4
Quantitation of the RNA polymerase II transcription machinery in yeast.
J Biol Chem. 2001 Dec 14;276(50):47150-3. doi: 10.1074/jbc.M109581200. Epub 2001 Oct 8.
5
The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II.Swi5激活剂在没有RNA聚合酶II的情况下将中介复合物募集到HO启动子上。
Genes Dev. 2001 Sep 15;15(18):2457-69. doi: 10.1101/gad.921601.
6
Mediator, not holoenzyme, is directly recruited to the heat shock promoter by HSF upon heat shock.热休克时，HSF将介质而非全酶直接募集至热休克启动子。
Mol Cell. 2001 Jul;8(1):9-19. doi: 10.1016/s1097-2765(01)00296-9.
7
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Biochemistry. 2001 Aug 7;40(31):9421-7. doi: 10.1021/bi010011k.
8
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