儿童对幽门螺杆菌和CagA抗原的IgG亚类反应。
IgG subclass response to Helicobacter pylori and CagA antigens in children.
作者信息
Dzierzanowska-Fangrat K, Raeiszadeh M, Dzierzanowska D, Gladkowska-Dura M, Celinska-Cedro D, Crabtree J E
机构信息
Molecular Medicine Unit, St James's University Hospital, Leeds, UK.
出版信息
Clin Exp Immunol. 2003 Dec;134(3):442-6. doi: 10.1111/j.1365-2249.2003.02304.x.
Specific serum IgG subclass antibodies against Helicobacter pylori antigens and recombinant CagA were analysed in 75 symptomatic children with histologically confirmed H. pylori infection. H. pylori stimulated an IgG1 predominant response, and IgG3 titres showed a positive association with peptic ulcer disease, chronicity of antral inflammation and density of H. pylori colonization. Two methods used for assessing serum IgG CagA antibody status, i.e. Western blotting and enzyme-linked immunosorbent assay (ELISA), were concordant. CagA stimulated an IgG1 and IgG3 predominant humoral response. Total CagA IgG titres were higher in children with active and more severe chronic antral inflammation. These findings suggest that in children the systemic humoral immune response to H. pylori infection may reflect gastroduodenal pathology.
对75名经组织学确诊为幽门螺杆菌感染的有症状儿童，分析了针对幽门螺杆菌抗原和重组细胞毒素相关基因A（CagA）的特异性血清IgG亚类抗体。幽门螺杆菌刺激产生以IgG1为主的反应，IgG3滴度与消化性溃疡疾病、胃窦炎慢性化及幽门螺杆菌定植密度呈正相关。用于评估血清CagA抗体状态的两种方法，即蛋白质印迹法和酶联免疫吸附测定（ELISA），结果一致。CagA刺激产生以IgG1和IgG3为主的体液反应。在患有活动性和更严重慢性胃窦炎的儿童中，总CagA IgG滴度更高。这些发现表明，在儿童中，对幽门螺杆菌感染的全身体液免疫反应可能反映胃十二指肠病理情况。
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