掌腱膜挛缩症成纤维细胞对5α-双氢睾酮的反应性。
Responsiveness of Dupuytren's disease fibroblasts to 5 alpha-dihydrotestosterone.
作者信息
Pagnotta Alessia, Specchia Nicola, Soccetti Andrea, Manzotti Sandra, Greco Francesco
机构信息
Institute of Orthopedics, University of Ancona, Ancona, Italy.
出版信息
J Hand Surg Am. 2003 Nov;28(6):1029-34. doi: 10.1016/s0363-5023(03)00429-5.
PURPOSE
We recently showed that androgen receptors are expressed in Dupuytren's contracture. The aim of the present work was to test the responsiveness of Dupuytren's fibroblasts to 5 alpha-dihydrotestosterone (5 alpha-DHT), the active form of testosterone.
RESULTS
Cultured palmar fascia cells from 10 patients with Dupuytren's contracture and 4 normal subjects were exposed to 5 alpha-DHT (10 or 100 ng/mL) for 1, 3, 7, and 15 days. Their phenotype was analyzed immunohistochemically for alpha-smooth muscle actin and androgen receptor expression and proliferation rates were studied.
RESULTS
At 15 days the higher concentration of 5 alpha-DHT induced an increase in Dupuytren's fibroblast proliferation, whereas anti-alpha-smooth muscle actin exhibited the strongest expression. At the same time point androgen receptor expression decreased with the lower concentration and disappeared altogether with the higher dose of 5 alpha-DHT.
CONCLUSIONS
The palmar fascia is a target tissue for androgen action via androgen receptors. Further studies are required to determine whether control of androgen receptor may control the evolution of Dupuytren's disease.
目的
我们最近发现雄激素受体在掌腱膜挛缩症中表达。本研究的目的是测试掌腱膜成纤维细胞对睾酮的活性形式5α-二氢睾酮（5α-DHT）的反应性。
结果
将10例掌腱膜挛缩症患者和4例正常受试者的掌腱膜细胞培养物暴露于5α-DHT（10或100 ng/mL）中1、3、7和15天。通过免疫组织化学分析其α-平滑肌肌动蛋白和雄激素受体表达的表型，并研究增殖率。
结果
在15天时，较高浓度的5α-DHT诱导掌腱膜成纤维细胞增殖增加，而抗α-平滑肌肌动蛋白表现出最强的表达。在同一时间点，较低浓度的5α-DHT使雄激素受体表达降低，而较高剂量的5α-DHT使其完全消失。
结论
掌腱膜是雄激素通过雄激素受体发挥作用的靶组织。需要进一步研究以确定雄激素受体的调控是否可控制掌腱膜挛缩症的发展。
Zotero 插件