植物乳杆菌WCFS1菌株的原噬菌体序列。

The prophage sequences of Lactobacillus plantarum strain WCFS1.

作者信息

Ventura Marco, Canchaya Carlos, Kleerebezem Michiel, de Vos Willem M, Siezen Roland J, Brüssow Harald

机构信息

Nestle Research Center, CH-1000 Lausanne 26, Vers-chez-les-Blanc, Switzerland.

出版信息

Virology. 2003 Nov 25;316(2):245-55. doi: 10.1016/j.virol.2003.08.019.

DOI:10.1016/j.virol.2003.08.019

PMID:14644607

Abstract

The Lactobacillus plantarum commensal WCFS1 contains four prophage elements in its genome. Lp1 and Lp2 are two about 40-kb-long uninducible prophages that share closely related DNA packaging, head and tail genes defining a second lineage of pac-site Siphoviridae in L. plantarum, distinct from L. plantarum phage phig1e, but related to Bacillus phage SPP1 and Lactococcus phage TP901-1. Northern analysis revealed transcribed prophage genes exclusively near both attachment sites. Comparative genomics identified candidate lysogenic conversion genes (LCG) downstream of the lysis cassette and within the lysogeny module. Notable are genes with sequence similarities to putative LCG from Streptococcus pyogenes prophages and to a Bacillus plasmid. Both prophages harbored tRNA genes. R-Lp3 and R-Lp4 represent short prophage remnants; R-Lp3 abuts Lp2 and displays sequence links to cos-site Siphoviridae.

摘要

植物乳杆菌共生菌WCFS1的基因组中含有四个原噬菌体元件。Lp1和Lp2是两个长度约为40 kb的不可诱导原噬菌体，它们具有密切相关的DNA包装、头部和尾部基因，这些基因定义了植物乳杆菌中pac位点长尾噬菌体科的第二个谱系，与植物乳杆菌噬菌体phig1e不同，但与芽孢杆菌噬菌体SPP1和乳酸乳球菌噬菌体TP901-1相关。Northern分析显示，仅在两个附着位点附近有转录的原噬菌体基因。比较基因组学确定了裂解盒下游和溶原模块内的候选溶原转化基因（LCG）。值得注意的是，这些基因与化脓性链球菌原噬菌体的推定LCG以及芽孢杆菌质粒具有序列相似性。这两个原噬菌体都含有tRNA基因。R-Lp3和R-Lp4代表短的原噬菌体残余物；R-Lp3邻接Lp2，并显示出与cos位点长尾噬菌体科的序列联系。