[龙胆属一些物种中18S - 25S核糖体基因的限制性图谱分析及变异性]

[Restriction mapping and variability of 18S-25S ribosomal genes in some species of Gentiana genus].

作者信息

Mel'nyk V M, Andrieiev I O, Spiridonova K B, Kunakh V A

出版信息

Tsitol Genet. 2003 Sep-Oct;37(5):65-71.

Abstract

Mapping of rRNA genes in four species of Gentiana genus has been carried out by blot-hybridization method using some restriction endonucleases. The following characteristics of Gentiana rDNA structural organization have been revealed: 1) interspecific and intragenomic variability of the length of ribosomic repeats; 2) conservativity of the transcribed region; 3) variability of the length and location of Hind-III site in non-transcribed spacer; 4) interspecific variability of the number of copies. Comparative analysis of the constructed restriction maps of Gentiana species and some other plants revealed the similarity of restriction site location in the transcribed DNA region.

摘要

利用一些限制性内切酶，通过印迹杂交法对龙胆属四个物种的rRNA基因进行了定位。已揭示出龙胆属rDNA结构组织的以下特征：1）核糖体重复序列长度的种间和基因组内变异性；2）转录区域的保守性；3）非转录间隔区中Hind-III位点长度和位置的变异性；4）拷贝数的种间变异性。对构建的龙胆属物种和其他一些植物的限制性图谱进行比较分析，揭示了转录DNA区域中限制性位点位置的相似性。