Millar B Cherie, Jiru Xu, Walker Michael J, Evans James P, Moore John E
Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, United Kingdom.
J Clin Microbiol. 2003 Dec;41(12):5778-80. doi: 10.1128/JCM.41.12.5778-5780.2003.
rRNA sequence analysis of a partial region of the 18S and 5.8S-internal transcribed spacer 2 (ITS2) region of Chrysosporium keratinophilum highlights its potential molecular misidentification as Coccidioides immitis. Molecular identification of medically important fungi should not be based solely on sequence analysis of the 18S rRNA gene but should be confirmed by sequence analysis of an additional rRNA gene locus, such as the ITS region(s).
嗜角质金孢子菌18S和5.8S-内转录间隔区2(ITS2)区域部分片段的rRNA序列分析突显了其可能被误鉴定为粗球孢子菌的情况。对具有医学重要性的真菌进行分子鉴定时,不应仅基于18S rRNA基因的序列分析,而应以额外的rRNA基因位点(如ITS区域)的序列分析来加以确认。