p53、bax、bcl-2和p21在结直肠癌中表达诱导细胞凋亡。
Induction of apoptosis by p53, bax, bcl-2, and p21 expressed in colorectal cancer.
作者信息
Katsumata Kenji, Sumi Tetsuo, Tomioka Hidenori, Aoki Tatsuya, Koyanagi Yasuhisa
机构信息
The Department of Surgery, Tokyo Medical University, Tokyo, Japan.
出版信息
Int J Clin Oncol. 2003 Dec;8(6):352-6. doi: 10.1007/s10147-003-0352-6.
BACKGROUND
We investigated the influence of genes on the apoptosis of colorectal tumor cells, based on DNA and mRNA kinetics.
METHODS
In 30 colorectal cancer patients, we examined the mRNA expression of p53, bax, bcl-2, and p21(WAF1), and we also investigated the development of tumor cell apoptosis, using a terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) method.
RESULTS
TUNEL-positive cells showed a positive correlation with bax (P = 0.010) and a negative correlation with p21 (P = 0.04). We also investigated the relationship between p53 point mutation, p21 immunostaining degree, and apoptosis, based on DNA ladder expression. A remarkable correlation (P = 0.0090) was found between p21 and apoptosis.
CONCLUSIONS
The present study findings suggest that tumor cell apoptosis is (1) strongly inhibited by p21, (2) induced by bax, and (3) influenced by bcl-2, which, presumably, inhibits tumor cell apoptosis.
背景
基于DNA和mRNA动力学，我们研究了基因对结直肠肿瘤细胞凋亡的影响。
方法
在30例结直肠癌患者中，我们检测了p53、bax、bcl-2和p21（WAF1）的mRNA表达，并使用末端脱氧核苷酸转移酶（TdT）介导的dUTP-生物素缺口末端标记（TUNEL）法研究了肿瘤细胞凋亡的发生情况。
结果
TUNEL阳性细胞与bax呈正相关（P = 0.010），与p21呈负相关（P = 0.04）。我们还基于DNA梯带表达研究了p53点突变、p21免疫染色程度与凋亡之间的关系。发现p21与凋亡之间存在显著相关性（P = 0.0090）。
结论
本研究结果表明，肿瘤细胞凋亡（1）受到p21的强烈抑制，（2）由bax诱导，（3）受到bcl-2的影响，推测bcl-2抑制肿瘤细胞凋亡。
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